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To create an image with transparent background open
Figure Properties or
Fit Plot Properties dialog window and uncheck
Fill Canvas box in
The background of transparent Figures and Fit Plots are painted by grey chequerboard. If you are preparing a picture for a dark background you can change the colour of chequerboard to dark by selecting
View → Transparency Checkerboard in main menu. The possible choices are the light (default), medium and dark grey.
In case of transparent background you can set the preview background colour in
Image Preview window.