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To create an image with transparent background open Figure Properties
or Fit Plot Properties
dialog window and uncheck Fill Canvas
box in Canvas
tab.
The background of transparent Figures and Fit Plots are painted by grey chequerboard. If you are preparing a picture for a dark background you can change the colour of chequerboard to dark by selecting View → Transparency Checkerboard
in main menu. The possible choices are the light (default), medium and dark grey.